L-Selectin anticorps (APC)

N° du produit ABIN6657789

N° du produit (Fournisseur): 200-526-n87

Cet anticorps Rat Monoclonal détecte spécifiquement L-Selectin dans FACS, IHC et IP. Il présente une réactivité envers Souris.

Aperçu rapide pour L-Selectin anticorps (APC) (ABIN6657789)

Antigène: L-Selectin (SELL) (Selectin L (SELL))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp L-Selectin est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: MEL-14

Détail du produit anti-L-Selectin anticorps (APC)

N° du produit (Fournisseur): 200-526-n87

Fournisseur: Rockland

Fonction: CD62L Allophycocyanin Antibody

Réactivité croisée (Details): Cross reactivity with CD62L from other sources has not been tested.

Purification: Allophycocyanin conjugated CD62L Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD62L.

Stérilité: Sterile filtered

Immunogène: Anti-CD62L Antibody (Monoclonal) was produced by repeated immunizations with CD62L antigen.

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Commentaires:

Anti-CD62L is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Date de péremption: 6 months

Détails sur L-Selectin

Antigène: L-Selectin (SELL) (Selectin L (SELL))

Autre désignation: CD62L

Sujet:

Synonyms: L-selectin CD62 antigen-like family member L Leukocyte adhesion molecule 1 LAM-1 Leukocyte-endothelial cell adhesion molecule 1 LECAM1 Lymph node homing receptor Lymphocyte antigen 22 Ly-22 Lymphocyte surface MEL-14 antigen CD62L Sell Lnhr, Ly-22, Ly22

Background: Anti-CD62L antibody is specific for mouse CD62L, also known as L-Selectin, a cell adhesion molecule which facilitates lymphocyte rolling on activated vascular endothelium and homing to high endothelial venules (HEV) as immune cells transmigrate from blood into peripheral tissues. L-Selectin is a member of a family of Selectin molecules which act together with the integrin family of adhesion molecules to mediate leukocyte-endothelial interactions. L-Selectin is characteristically expressed by neutrophils, and is also found on B cells, monocytes, granulocytes, and at varying levels on naive, effector and memory T cells. It is rapidly shed upon cell activation, releasing into the circulation a soluble form whose biological role is of particular interest in cancer biology research.The MEL-14 antibody may be used as a phenotypic marker for CD62L expression on a variety of immune cell types. Please note that CD62L (L-Selectin) itself is also referred to as MEL-14 in the literature.

Gene Name: Sell

ID gène: 20343

NCBI Accession: NP_035476

UniProt: P18337